The number of laser in situ keratomileusis (LASIK) procedures is continuing to rise. Since its first application for correcting simple refractive errors over 25 years ago, the role of LASIK has extended to treat other conditions, including postkeratoplasty astigmatism/ametropia, postcataract surgery refractive error and presbyopia, among others. The long-term effectiveness, predictability and safety have been well established by many large studies. However, due to the creation of a potential interface between the flap and the underlying stroma, interface complications such as infectious keratitis, diffuse lamellar keratitis and epithelial ingrowth may occur. Post-LASIK epithelial ingrowth (PLEI) is an uncommon complication that usually arises during the early postoperative period. The reported incidence of PLEI ranged from 0%–3.9% in primary treatment to 10%–20% in retreatment cases. It can cause a wide spectrum of clinical presentations, ranging from asymptomatic interface changes to severe visual impairment and flap melt requiring keratoplasty. PLEI can usually be treated with mechanical debridement of the affected interface; however, additional interventions, such as alcohol, mitomycin C, fibrin glue, ocular hydrogel sealant, neodymium:yttriumaluminum garnet laser and amniotic membrane graft, may be required for recurrent or refractory cases. The aims of this review are to determine the prevalence and risk factors of PLEI; to describe its pathogenesis and clinical features and to summarise the therapeutic armamentarium and the visual outcome of PLEI.
- ocular surface
- treatment lasers
- treatment surgery
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The history of modern-day laser in situ keratomileusis (LASIK) dates back to 1985 when Peyman first introduced the concept of performing laser ablation under a corneal flap.1 In 1990, Pallikaris et al 2 performed the first LASIK procedure in a rabbit model using a modified microkeratome for corneal flap creation coupled with excimer laser. To date, LASIK remains the most widely performed laser refractive surgery worldwide, with over 1.2 million LASIK surgeries being performed annually in the USA and Europe.3
Since its introduction, the role of LASIK has expanded from the initial correction of simple refractive errors to other clinical and refractive indications, including the management of postkeratoplasty astigmatism/ametropia, postcataract surgery refractive error and presbyopia, among others.4–7 The long-term effectiveness, predictability and safety of LASIK have been well established by many long-term studies.8 9 A recent systematic review of LASIK in 67 893 eyes reported excellent visual and refractive outcomes with very high patient satisfaction following modern LASIK surgery; 99.5% of patients achieved uncorrected distance visual acuity of 6/12 or better and 98.6% of patients achieved spherical equivalent refraction within ±1.0 dioptre (D) of target refraction.10
Nonetheless, the creation of an interface between the flap and the underlying corneal stroma can lead to flap-related and flap-stromal interface complications such as flap dislocation, infectious keratitis, diffuse lamellar keratitis and epithelial ingrowth (EI). Post-LASIK epithelial ingrowth (PLEI) is a rare complication that is characterised by the ingrowth of corneal epithelium at the interface between the flap and stromal bed following LASIK, leading to a range of symptoms and signs. In view of its low prevalence, the experience of treating EI is largely based on anecdotal case reports and small case series. The aims of this review are threefold: first, to determine the prevalence and risk factors of PLEI; second, to describe the pathogenesis and clinical features of EI and third, to summarise the therapeutic armamentarium and the visual outcome of PLEI.
Prevalence of epithelial ingrowth following LASIK and other types of surgery
The prevalence of EI following primary LASIK is very low, estimated between 0% and 3.9%.11–15 However, the risk of EI is significantly increased in various clinical circumstances, especially when the flap is lifted for retreatment (up to 10%–20%).16–18 EI has also been rarely reported following trauma and other types of ocular surgery, including penetrating trauma, keratoplasty, cataract and pterygium surgery.19–21 Such manifestation is due to the inadvertent introduction of corneal epithelial cells into the intraocular environment, resulting in proliferation of epithelial cells on the surface of intraocular structures such as posterior cornea, anterior chamber angle, ciliary body, iris and lens capsule.
Risk factors of post-LASIK epithelial ingrowth
Although the risk of PLEI is low, clinical management can be very challenging in some cases. The risk factors for PLEI are summarised in box 1. A thorough understanding of these risk factors enables better preoperative patient counselling and risk assessment.
Modifiable and non-modifiable risk factors of epithelial ingrowth following LASIK
Modifiable risk factors
m-LASIK>f-LASIK in risk of PLEI following primary and enhancement treatment
Surgical technique during retreatment
Direct flap lift with forceps>cleaving with spatula before lifting
Conformation of LASIK flap edge
70° side cut>115° side cut
Corneal epithelial injury
LASIK flap dislocation
Non-modifiable risk factors
Increased age (weak evidence)
Type 1 diabetes mellitus
Corneal epithelial basement membrane dystrophy or recurrent corneal erosion syndrome
Types of refractive correction
Hyperopic LASIK > myopic LASIK
Flap lift for retreatment
PLEI, post-LASIK epithelial ingrowth; LASIK, laser in situ keratomileusis; m-LASIK, microkeratome-assisted LASIK; f-LASIK, femtosecond LASIK.
Several modifiable factors of PLEI have been described in the literature; these include surgical instrumentation, retreatment technique and conformation of LASIK flap hinge.
Flap creation by femtosecond laser or microkeratome represents one of the major steps in LASIK surgery. Some studies have suggested that the instruments used during flap creation could influence the risk of PLEI.18 22 23 Letko et al 18 observed that the risk of EI following LASIK flap-lift for retreatment was significantly lower in patients who were initially treated with femtosecond LASIK (f-LASIK; 1.4%) as compared with microkeratome-assisted LASIK (m-LASIK; 8.3%). In another study of 6415 eyes that underwent f-LASIK, the risk of PLEI was reported to be as low as 0.03%.23 It was postulated that the lower incidence of PLEI following f-LASIK was attributed to the geometry of the flap edge and the lower risk of peripheral trauma at the flap edge.
Surgical technique during LASIK retreatment
Although the long-term efficacy and stability of LASIK surgery have been reported in the published literature, retreatment is occasionally required in some cases due to regression or inadequacy of the treatment effect.24 25 Flap lift for retreatment is a well-recognised risk factor for PLEI. Nonetheless, Chan and Boxer Wachler26 observed that using a Pinelli spatula to cleave the circumference of the flap edge before lifting the flap could significantly reduce the risk of PLEI when compared with direct flap lifting using forceps. It was suggested that the use of spatula reduced the risk of trauma to the corneal epithelium at the flap edge. On the other hand, the risk of EI associated with LASIK retreatment could be eliminated by employing photorefractive keratectomy over the previous LASIK flap as a retreatment strategy, obviating the need of flap lifting for retreatment.16
Conformation of LASIK flap edge
The conformation of LASIK flap edge may also have an effect on the risk of PLEI. In a rabbit model, Jhanji et al 27 reported that PLEI was more frequently observed in flaps with 70° (conventional) side cuts compared with flaps with 115° (inverted) side cuts. In addition, corneas with 70° side cuts showed significantly increased apoptotic cells compared with the inverted side-cuts corneas. Asano-Kato et al 28 similarly reported a greater incidence of PLEI using MK-2000 microkeratome (Nidek, Aichi, Japan) compared with LSK-One microkeratome (Moria, Antony, France) due to the difference in flap edges.
Corneal epithelial injury
Asano-Kato et al 28 and Jabbur et al 29 have reported that intraoperative epithelial injury/defect is a risk factor of PLEI. Similarly, Wang and Maloney15 observed that 33% of their patients who had a postoperative epithelial defect subsequently developed PLEI. They observed that patients with epithelial basement membrane dystrophy had a higher recurrence rate of PLEI, requiring multiple surgical debridement. They proposed that the epithelial defect resulted in flap hydration/oedema, ultimately leading to a poor adhesion of the flap-stromal interface with PLEI.
LASIK flap dislocation
LASIK flap dislocation is a recognised risk for PLEI.30 31 It shares the similar principle with cases of flap-lift retreatment where the corneal epithelial cells at the flap-host junction are given access to the flap-stromal interface due to poor adhesion/dislocation of the flap edge. Therefore, the development of late-onset PLEI should raise the suspicion of occult dislocation of the LASIK flap and a history of trauma should be elicited.31
Several non-modifiable factors have also been described in the literature. These include patient demographics, preoperative refractive errors and primary and retreatment procedures.
One study of 30 eyes suggested that older age might increase the risk of PLEI.26 However, this was not observed in a larger study that included 4500 LASIK eyes.12 Similarly, there is no clear gender or race predilection for PLEI.12 In terms of ocular factors, various studies have demonstrated the positive association between PLEI and corneal epithelial basement membrane dystrophy.15 29 Systemic factor such as type 1 diabetes mellitus may also increase the risk of PLEI.29
Hyperopic versus myopic LASIK
Hyperopic LASIK has been associated with a higher risk of PLEI as compared with myopic LASIK.11 32 In a retrospective study of 1000 m-LASIK procedures, Mohamed et al 11 had observed a significant higher risk of PLEI in patients who were undergoing hyperopic LASIK as opposed to myopic LASIK. The incidence of PLEI following hyperopic LASIK was seven times higher than myopic LASIK (23% vs 3%) and the increased risk was observed in both primary (17% vs 3%) and enhancement treatment (43% vs 7%).11 This may be related to the fact that laser ablation is applied at the mid-periphery of the cornea in hyperopic LASIK as opposed to the central of the cornea in myopic LASIK. The conformational change in the mid-periphery of the cornea (closer to the flap edge) can potentially affect the flap-stromal adhesion, increasing the risk of PLEI.
Primary versus retreatment LASIK
Although LASIK surgery has a well-established long-term effectiveness and refractive stability, approximately up to 10%–14% of the patients may undergo retreatment/enhancement at some stage following their initial LASIK surgery.15 33–35 LASIK flap lifts for retreatment increases the risk for EI with the reported incidence ranging from 1.7% to 23.3% when compared with 0% to 3.9% for primary treatments.11–18 33 Moreover, flap-lift retreatment performed three or more years after primary LASIK carry a higher risk of clinically significant PLEI compared with retreatments with flap lifts done earlier.12
Pathogenesis of post-LASIK epithelial ingrowth and corneal wound healing
The pathogenesis of PLEI is attributed to two underlying mechanisms. Implantation of the corneal epithelial cells during the flap creation, resulting in epithelial nests away from the flap edge. These epithelial cells usually have minimal proliferative ability due to limited nourishment from the ocular surface environment and may remain asymptomatic. Second, PLEI may be a consequence of corneal epithelial cells migrating from the flap edge towards the flap-stromal interface due to poor flap adhesion, flap dislocation or presence of foreign bodies (eg, blood, cell infiltrate, ointment, etc) at the flap-stromal interface.15 28 In this instance, the poorly attached or dislocated flap allows the invasion of epithelial cells into the interface. Subsequently, these invaded cells lift up the edge of the flap, causing pooling of the fluorescein.15 Proliferation of the invaded epithelial cells culminates in the formation of a fistula, resulting in progressive PLEI. Interestingly, the histopathological findings of PLEI differs in early and late stages according to a Japanese study.36 It was shown that early PLEI comprised multilayered squamous epithelium resembling normal corneal epithelium, whereas late PLEI consisted of clumps containing amorphous materials with little cellular elements, potentially reflecting different proliferative activity of EI at different stages.36
Various studies have shown that the corneal wound healing following LASIK primarily occurs at the periphery of the LASIK flap-corneal stromal junction.37 38 This healing phenomenon explains the late onset of LASIK flap dislocation, even over a decade after the initial LASIK surgery.31 Therefore, the development of late-onset of PLEI in a patient with previous LASIK surgery should raise the suspicion of occult traumatic dislocation of LASIK flap.
Based on the location, clinical features and severity, PLEI can be categorised into four grades using the Prosbt/Machat classification (table 2).39 This classification serves as a useful aid for consistent and standardised grading/assessment and provides guidance on the treatment strategies.
Clinical features and assessment
A wide spectrum of clinical presentations has been reported in the literature; these range from asymptomatic corneal changes to severe visual impairment and flap melt requiring keratoplasty. The onset of PLEI usually takes place within a month following primary or enhancement LASIK.15 However, many patients are asymptomatic from PLEI, explaining the wide range of time interval between the initial LASIK surgery and presentation of PLEI (0.5–108 months).40 Rapuano reported in his study that as many as 64% of the eyes affected by PLEI did not require any intervention.40 These patients usually have PLEI within 2 mm from the flap edge (Prosbt/Machat grade 1) with no visual symptoms. In some cases, patients may experience foreign body sensation and glare due to ocular surface irregularity secondary to PLEI.
In terms of clinical features, Wang and Maloney15 have described four key clinical signs/characteristics that could aid in the diagnosis of PLEI. These include epithelial Pearls/nest in the interface (figure 1A), fluorescein pooling at the edge of the flap (due to elevation of flap edge by underlying invading epithelial cells), white fibrotic demarcation line and keratolysis/melting of the flap edge. Other signs include diffuse opaque epithelial ingrowth (figure 1B), rolled up flap edge with thickened whitish-grey appearance, staining channels and peripheral confluent haze.
In addition to slit lamp examination, other investigative tools can be used to assist in the assessment and monitoring of PLEI. Anterior segment optical coherence tomography serves as a useful investigation in detecting subtle PLEI changes that are not readily evident on slit lamp examination (figure 1C-D). Corneal topography/tomography can be used to assess the PLEI-induced refractive changes and ocular surface irregularity. In addition, corneal densitometry using Scheimpflug tomography can be used to provide objective quantification and monitoring of the severity and progression of EI.41
The indications for medical or surgical treatment of PLEI are largely guided by the patient’s symptoms, location and severity of the PLEI, and the presence of any associated pathology such as flap dislocation/dehiscence. In most cases, PLEI can be surgically removed with mechanical debridement of the interface; however, clinically significant recurrence may be as high as 36% after such treatment.42 The visual outcomes, recurrence rate and complication of various treatment modalities of PLEI are summarised in table 3.13 15 40 42–60
The most common treatment strategy for PLEI is mechanical debridement of the EI from the flap-stromal interface and from the inner side of the flap followed by careful repositioning of the flap and avoidance of any intraoperative/postoperative epithelial trauma.15 40 42 Flap suturing, fibrin glue and ocular hydrogel sealant at the flap edge have been proposed to facilitate secured flap adhesion.13 40 43 46–53 In refractory cases, various adjunctive therapies can be used to help eradicate PLEI. Amniotic membrane graft (AMG) may be used in cases of PLEI associated with flap injury or melting.54–56 In addition, the use of non-invasive neodymium:yttrium aluminum garnet (Nd:YAG) laser has also been reported as a novel technique for treating PLEI.58–60
Based on the available evidence in the literature, we recommend simple mechanical debridement in the first presentation of PLEI. Mechanical debridement can be performed using non-shredding spears, spatula or other appropriate instruments. A bandage contact lens should be placed to protect the flap and reduce the risk of flap dehiscence/dislocation,15 40 50 although there was a small study of 30 eyes reporting an increased risk of PLEI with bandage contact lens use.26 All patients should be counselled on the risk of recurrence of EI (0%–36%) following the treatment, which may require further debridement. In patients with one or more episodes of recurrent EI, adjunctive therapies need to be administered to reduce further risk of recurrence. Various strategies have been described in the literature but many of the studies are of anecdotal case reports and small case series. Based on the evidence from larger studies (≥10 treated eyes), we recommend the use of either flap suturing with 10’0 nylon or fibrin glue to achieve secured flap-stromal adhesion. The recurrence rates following these treatment strategies range between 0%–33% and 0%–7.7%, respectively. The low recurrence rate of PLEI following the application of fibrin glue is likely attributed to its inhibitory property on the migration of ocular surface epithelial cells.61 In addition, antimetabolite, such as mitomycin C 0.02%, and AMG can be used to treat refractory cases and those associated with flap melt/injury, respectively. For patients who are unable to tolerate surgery, Nd:YAG laser using low energy of (0.6 mJ on average) can be applied as an alternative treatment. However, the experience and evidence is limited to one large case series of 30 eyes and a few case reports, and care needs to be taken during the procedure to avoid any flap complication. In addition, 40% or more patients require repeat treatment to eliminate the EI completely.
The most common complication following the treatment of PLEI is recurrence of EI, usually recurring at the original area of PLEI. Risk factors for recurrence include infectious aetiology of EI, use of microkeratome for primary LASIK, hyperopic LASIK and previous history of recurrence.40 42 43 Based on large case series, the recurrence rate following treatment, depending on the treatment strategy and follow-up duration, ranged between 0% and 36% (table 3). Mechanical debridement of both the interface and the inner aspect of the flap followed by the application of fibrin glue appears to have the lowest recurrence rate of PLEI as compared with other treatment modalities. Other uncommon complications such as diffuse lamellar keratitis and flap melt (up to 80% of the flap) have been reported following the use of highly concentrated ethanol (50%–100%) at the flap-stromal interface.6 62 Non-invasive Nd:YAG laser treatment of PLEI may also lead to localised flap melt, flap surface breakthrough and inadvertent creation of new channel at the flap edge, resulting in new areas of EI.63 64
Visual outcome and prognosis
The visual outcome of PLEI is largely dependent on the location and the severity of the ingrowth (Probst/Machat classification). However, PLEI is often not graded according to the classification, but by the need for intervention, in many studies (eg, whether PLEI is clinically significant to warrant removal). Therefore, by definition, patients who require surgical/procedural intervention have PLEI of grade 2 or more. In addition, the visual outcome may be confounded by publication bias (eg, only studies with good visual outcome are published).
Patients with grade 1 PLEI (within 2 mm of flap edge without any problem with the flap edge) are usually asymptomatic with unaffected vision. Rapuano has reported that 64% of his patients with PLEI were managed with simple observation.40 In grade 2 and grade 3 PLEI, good visual outcome is normally retained following appropriate treatment. Based on larger studies (≥10 treated eyes), patients with PLEI usually achieved uncorrected distance visual acuity of ≥6/12 (in 74%–80% cases) and ≥6/7.5 (in 45%–53% cases), and corrected distance visual acuity of ≥6/12 (in 77%–91% cases) and ≥6/7.5 (in 78%–85% cases) following treatment. Central involvement of the cornea serves as the poor visual prognostic factor for PLEI.42 In extreme cases of flap melt (grade 4), significant flap-stromal interface scarring or aggressive PLEI, flap amputation or penetrating keratoplasty may be required to improve the vision and eradicate persistent PLEI.65–67 On the other hand, a rare case of spontaneous resolution of PLEI has been reported in the literature.68
With the continuing rise of LASIK procedures, it is important for all ophthalmologists to become competent in recognising and/or managing post-LASIK complications, including PLEI. Understanding of both modifiable and non-modifiable risk factors helps reduce the incidence of PLEI and also enables better patient counselling preoperatively. When appropriately managed, good visual prognosis is usually achieved in patients with PLEI. However, additional measures and interventions may be required in aggressive or refractory PLEI cases in order to achieve complete and permanent eradication of the disease.
Method of literature search
A literature search in PubMed electronic database was conducted to identify all articles concerning EI following LASIK. Abstracts were further reviewed and the reference lists were hand-searched to ensure complete inclusion of all relevant articles. Only articles published in English were included. Certain keywords, including epithelial ingrowth, epithelial downgrowth, laser in situ keratomileusis and LASIK, were used during the literature search.
Contributors DSJT is involved in the conception and planning of the study, review of the literature, manuscript drafting and final approval of the manuscript. SS is involved in the review of the literature, critical appraisal and final approval of the manuscript. J-PD is involved in the review of the literature, critical appraisal and final approval of the manuscript.
Funding This research received no specific grant from any funding agency in the public, commercial or not-for-profit sectors.
Competing interests None declared.
Patient consent Detail has been removed from this case description/these case descriptions to ensure anonymity. The editors and reviewers have seen the detailed information available and are satisfied that the information backs up the case the authors are making.
Provenance and peer review Not commissioned; externally peer reviewed.
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