Abstract
A patient had corneal infection with Staphylococcus aureus after keratoplasty from tissue preserved in McCarey-Kaufman medium. The small, successfully treated corneal infection was from the same organism cultured from the tissue culture medium at the time of transplantation. Possible sources of donor-tissue contamination included the increase in ocular flora seen in postmortem eyes, blood-borne bacteria, lack of sterile technique in securing donor tissue, inadequate irrigation of the tissue, and inadequate use of topical antibiotics on the tissue. Preservation medium and specimens from medium-preserved and refrigerated corneas should be cultured before and during surgery.
Publication types
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Case Reports
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Administration, Topical
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Anti-Bacterial Agents / pharmacology
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Bacitracin / administration & dosage
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Bacitracin / therapeutic use
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Cornea / microbiology
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Corneal Transplantation*
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Culture Media / standards*
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Drug Contamination*
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Female
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Gentamicins / pharmacology
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Humans
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Injections, Intravenous
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Keratitis / drug therapy
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Keratitis / etiology*
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Methicillin / administration & dosage
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Methicillin / therapeutic use
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Microbial Sensitivity Tests
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Middle Aged
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Postoperative Complications / drug therapy
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Postoperative Complications / etiology
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Staphylococcus aureus* / drug effects
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Staphylococcus aureus* / isolation & purification
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Transplantation, Homologous
Substances
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Anti-Bacterial Agents
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Culture Media
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Gentamicins
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Bacitracin
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Methicillin