Original articleFactors Influencing Outcomes in Cultivated Limbal Epithelial Transplantation for Chronic Cicatricial Ocular Surface Disorders
Section snippets
Patients
Twenty-seven eyes of 27 patients that had CLET between June 1999 and November 2003 were retrospectively analyzed in this study. Patients consisted of 16 males and 11 females, with a mean age of 50.2 ± 20.7 years (15 to 82 years). All eyes had total limbal dysfunction. Conjunctivalization was confirmed preoperatively by impression cytology in 10 of 27 eyes. Mean corrected visual acuity was counting fingers, and two-thirds of the eyes were lower than 20/2000. The Schirmer test was performed in 21
Histologic Examinations
Multilayered epithelial cells were reconstructed on AM in the epithelial sheets produced by both Methods 1 and 2. The cells in Method 2 gave rise to four to five layers of epithelial cells with cuboidal basal cells and flat superficial cells. The cells in Method 1 showed relatively poor polarity and rough surface (Figure 1). Immunohistochemistry for cornea-specific keratins (keratin 3 and 12) demonstrated that cultivated epithelial cells in both methods were corneal phenotype.
Influence of Cultivation Methods on Surgical Outcomes
Demographic
Discussion
There have been increasing numbers of studies of CLET in both clinical outcome and research aspects.1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26 Although short-term outcomes appeared promising, only a few mid-term or long-term results have been reported. Validity of CLET for acute phase of chemical burns and SJS was reported6; however, the efficacy of the method for chronic cicatricial keratoconjunctivitis seems to be less dramatic.7 In the present study, we
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2021, Experimental Eye ResearchLong-term outcomes of cultivated cell sheet transplantation for treating total limbal stem cell deficiency: Long-term outcomes of cultivated cell sheet transplantation
2020, Ocular SurfaceCitation Excerpt :We currently intensively treat epithelial defects prior to surgical intervention with various medical or surgical approaches, including artificial tears, mucin secretagogues, medical-use contact lenses, punctal plugs/surgical occlusions, semi- or full-scleral contact lenses, mucous membrane grafting to the lid margin, and AM transplantation, which potentially improve the clinical outcome of CCST. A characteristic of the present study was that many kinds of surgical methods were included, such as CLET [7,8,10,21,23,31–60] and COMET [13,19,45,61–75]. Because this was a single center study, various factors that may influence surgical outcomes such as the preparation methods of the epithelial sheets, surgical methods, and postoperative management were relatively standardized.
Recovering vision in corneal epithelial stem cell deficient eyes
2019, Contact Lens and Anterior Eye