Table 3

Isolates from CIMs of cases of presumed MK

Isolates, n (%) (N=88)
Bacteria*
Total Gram-positive71 (80.7%)
 Staphylococcus aureus7 (8.0%)
 Streptococcus pneumoniae4 (4.5%)
 Other Streptococcus spp†11 (12.5%)
 CNS‡26 (29.5%)
 Other Gram-positive bacteria§23 (26.1%)
Total Gram-negative9 (10.2)
 Morganella morganii3 (3.4%)
 Enterobacter cloacae1 (1.1%)
 Serratia marcescens1 (1.1%)
 Klebsiella variicola1 (1.1%)
 Citrobacter youngae1 (1.1%)
 Sphingomonas paucimobilis1 (1.1%)
 Aeromonas caviae1 (1.1%)
Fungi¶0
Viral
 HSV-1**8 (9.1%)
Protozoa
 Acanthamoeba**0

  • *Bacterial isolates identified using culture and MALDI-TOF.

  • †Includes: Streptococcus oralis (4), Streptococcus gordonii (1), Streptococcus mitis (3), Enterococcus faecium (2), Streptococcus sanguinis (1).

  • ‡CNS, Includes: Staphylococcus epidermidis (19), Staphylococcus hominis (3), Staphylococcus haemolyticus (2), Staphylococcus nepalensis (1), Staphylococcus warneri (1).

  • §Includes: Bacillus sp (5), Diphtheroid sp (7), Micrococcus sp (8), Rothia mucilaginosa (1), Paenibacillus glucanolyticus (1), Kocuria marina (1).

  • ¶Fungal isolates identified using culture and MALDI-TOF.

  • **HSV-1 and Acanthamoeba isolates identified using targeted PCR.

  • CIM, corneal impression membrane; CNS, coagulase-negative Staphylococcus; HSV-1, herpes simplex virus type 1; MALDI-TOF, matrix-assisted laser desorption ionization-time-of-flight mass spectrometry; MK, microbial keratitis.