Table 1

Identified organisms by culture, smear and gene sequencing in each case

Case (Sample)CultureSmearGene sequencingReads (%)
1
(Vitreous)
Staphylococcus epidermidisStaphylococcus epidermidis672 (58.8)
Staphylococcus saccharolyticus329 (28.8)
Staphylococcus capitis50 (4.4)
Staphylococcus hominis22 (1.9)
Staphylococcus caprae16 (1.4)
2(Cornea)Moraxella sppGNRMoraxella nonliquefaciens1662 (95.5)
Moraxella catarrhalis40 (2.3)
Moraxella lacunata18 (1.0)
3 (Cornea)Staphylococcus aureusStaphylococcus aureus4496 (92.3)
Staphylococcus petrasii81 (1.7)
4 (Cyst)Haemophilus influenzaeGNRHaemophilus influenzae3261 (85.1)
Haemophilus aegyptius431 (11.2)
Cutibacterium acnes42 (1.1)
5 (Cornea)Pseudomonas aeruginosaGNRPseudomonas aeruginosa2659 (48.6)
Peptoniphilus lacydonensis362 (6.6)
Cutibacterium acnes347 (6.3)
Staphylococcus epidermidis274 (5.0)
Ralstonia pickettii248 (4.5)
Streptococcus pneumoniae244 (4.5)
Streptococcus dysgalactiae224 (4.1)
Finegoldia magna209 (3.8)
Staphylococcus saccharolyticus148 (2.7)
Staphylococcus aureus136 (2.5)
Streptococcus mitis100 (1.8)
6 (Cornea)Pseudomonas aeruginosaGNRPseudomonas aeruginosa17 (44.7)
CandidaRalstonia pickettii14 (36.8)
Sphingomonas leidyi2 (5.3)
NCRalstonia pickettii10 (83.3)
  • In the 16S rRNA gene analysis, low-abundance taxa with less than 1% of classified reads were discarded to minimise potential misidentification. Spurious classification results with a single mapped read were also eliminated.

  • GNR, gram-negative rods; NC, negative control; 16S rRNA, 16S ribosomal RNA .