Table 1

Endothelial cell viability in 20 paired, preloaded donor grafts for Descemet membrane endothelial keratoplasty (DMEK) shipped for 96 hours. Both tri-folded and scrolled DMEK grafts demonstrated comparable and acceptable rates of endothelial cell viability

DonorAgeDeath-to-preservation time (hours:min)Death-to-preparation time (days)ScrollTri-foldedStorage medium brand
ECD (cells/mm2, prior to peeling)Total ECL (%, after shipping)ECD (cells/mm2, prior to peeling)Total ECL (%, after shipping)
14714:1117328911.2316521.2Life4C
26706:223272511.4270315.6Life4C
36407:262285712.529335.7Optisol-GS
44405:0613290711.129338.7Life4C
56315:159199618.6170612.2Life4C
66816:105257711.7258417.2Life4C
76903:268289014.4284110.8Optisol-GS
86916:019196913.4177621.7Optisol-GS
97413:12330218.7303023.3Life4C
106807:5615203323.9207911.8Life4C
Average6310:308.4262613.7257514.8
  • ECD, Endothelial Cell Density; ECL, endothelial cell loss.