In vivo ocular surface characteristics during the 10 min period following instillation of undiluted MHME (1:10) or saline control to rabbit eyes. Data are presented as median (range). Asterisks denote statistically significant differences (p<0.05).
| Time | MHME | Saline control | p | |
| Conjunctival hyperaemia grade | Baseline | 0.500 (0.00-0.75) | 0.500 (0.00-0.75) | >0.99 |
| 30 s | 1.125 (1.00-1.5) | 0.500 (0.25-0.75) | <0.001* | |
| 5 min | 1.000 (0.75-1.25) | 0.625 (0.25-1.00) | <0.001* | |
| 10 min | 0.500 (0.50-1.00) | 0.500 (0.00-0.75) | 0.85 | |
| RM-ANOVA for treatment effect | 0.001* | |||
| RM-ANOVA for time effect | 0.002* | |||
| RM-ANOVA for interaction effect | <0.001* | |||
| Corneal opacity grade | Baseline | 0 (0–0) | 0 (0–0) | >0.99 |
| 30 s | 0 (0–0) | 0 (0–0) | >0.99 | |
| 5 min | 0 (0–0) | 0 (0–0) | >0.99 | |
| 10 min | 0 (0–0) | 0 (0–0) | >0.99 | |
| RM-ANOVA for treatment effect | >0.99 | |||
| RM-ANOVA for time effect | >0.99 | |||
| RM-ANOVA for interaction effect | >0.99 | |||
| Iris appearance grade | Baseline | 0 (0–0) | 0 (0–0) | >0.99 |
| 30 s | 0 (0–0) | 0 (0–0) | >0.99 | |
| 5 min | 0 (0–0) | 0 (0–0) | >0.99 | |
| 10 min | 0 (0–0) | 0 (0–0) | >0.99 | |
| RM-ANOVA for treatment effect | >0.99 | |||
| RM-ANOVA for time effect | >0.99 | |||
| RM-ANOVA for interaction effect | >0.99 | |||
| Ocular surface sodium fluorescein staining score | Baseline | 0 (0–0.250) | 0 (0–0) | 0.58 |
| 30 s | 0 (0–0.250) | 0 (0–0) | 0.58 | |
| 5 min | 0 (0–0.125) | 0 (0–0) | 0.94 | |
| 10 min | 0 (0–0.050) | 0 (0–0) | 0.99 | |
| RM-ANOVA for treatment effect | 0.36 | |||
| RM-ANOVA for time effect | 0.42 | |||
| RM-ANOVA for interaction effect | 0.42 |
MHME, Manuka honey microemulsion; RM-ANOVA, repeated measures analysis of variance.